IntroMOVIE 2: Time-lapse video of C. elegans development.

Brightfield images of 20 individuals are shown in a grid from hatching until young adulthood (see AIntroVID 5 for video of same animals from adulthood to death). Images were acquired at 5× magnification, using a custom culture apparatus (Zhang et al., 2016) maintained at 25°C. Specific life stages (L1-L4, pre-reproductive and reproductive adulthood) are denoted by the colored bars. These individuals are of genotype spe-9(hc88), and at the restrictive temperature of 25°C lay unfertilized oocytes. (Video source Z. Pincus.)