AIntroVID 5: Time-lapse video of group from adulthood to death.

Brightfield images of 20 individuals are shown in a grid from young adulthood until death (see IntroMOVIE 2 for video of same animals from hatching to adulthood). Images were acquired at 5× magnification, using a custom culture apparatus (Zhang et al., 2016) maintained at 25°C. Specific life stages (reproductive [egg] and post-reproductive [post] adulthood) are denoted by the colored bars; dead individuals fade to gray. These individuals are of genotype spe-9(hc88), and at the restrictive temperature of 25°C lay unfertilized oocytes.