PhaFIG 8A-G Legend

PhaFIG 8A-G: Gland cells of the pharynx.

There are four gland cells: two posterior subventral g2 cells, one syncytial right subventral-dorsal g1 cell, and a left subventral g1 cell.
A. Epifluorescent image of gland cells taken expressing the reporter gene B0280.7::GFP, left lateral oblique view. (Image source: R. Newbury.)
B. Graphic rendition of gland cells. The dorsal and right side g1 cells fuse at the anterior of the terminal bulb. The pair of subventral g1 cells open to the pharyngeal lumen at the posterior of the first bulb, whereas g2 gland cells open into the lumen anterior to the grinder. (Black ovals) Gland cell nuclei; (short arrows) gland openings to lumen.
C. The dorsal g1 gland duct lies within the dorsal nerve cord and opens to the lumen through a short cuticular duct (arrow) close to the buccal cavity. Nearby muscles attach to the duct with adherens junctions (white arrowhead). (Image source: [MRC] N2T (section A64-4).)
D & E. Epifluorescent images of gland cells expressing the reporter gene F20B10.1::GFP or phat-1::GFP (E, bottom panel). D. Ventral oblique view. (Arrow) Dorsal g1 process. E.Ventral view (both panels). (Arrows) Ventral g1 processes. (Strain source (top panel): L. Haklai-Topper and E. Peles; image source (bottom panel): R. Smit and J. Gaudet) Magnification, 400x.
F. The g1cells have lamellar cytoplasm, whereas the g2 cells have lighter cytoplasm with vesicles. TEM, transverse section through the posterior terminal bulb. (Image source: [MRC] N2W (section A420-16).)
G. Epifluorescent images of dorsal gland cells expressing the reporter gene Y8A9A.2::GFP. (Arrow) dorsal g1 process. (Image source: R. Smit, V.Ghai and J. Gaudet.)