The Pharynx of Caenorhabditis elegans - materiel and methods

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Table of contents  -  Abstract  -   Introduction  -   Materials & Methods  -   Results  -   Discussion  -   References

Material and Methods

Preparation and electron microscopy of 50 nm thick serial sections through Caenorhabditis elegans have been described (Ward et al. 1975; White et al. 1976, following paper).

The anatomy of the pharynx was reconstructed by tracing cell sections on electron micrographs in the same manner as described by Ward et al. (1975). A single series of one animal included the entire pharynx. Most cells in the pharynx are large and easily reconstructed from photographs taken of every third section, but in the region of the nerve ring in the metacorpus and where a commissure is found in the terminal bulb, additional series were necessary. At each of these places a series collected on slot grids was prepared so that all sections were visible for tracing neurone processes. Except in the middle of the procorpus, at least one additional partial series was cut for conformation of the findings. The distinctive morphology of neurones in the pharynx allows one to identify cell processes when their cell bodies are not included within a partial series. Further checks on assignments are made by comparison of bilaterally symmetric pairing of cells or cell processes.

Computer aided reconstruction from electron micrographs used in the preparation of some drawings has been described (White et al. 1976).

Web adaptation, Thomas Boulin, for Wormatlas, 2002, 2003