DPhaSUPFIG 1: Anterior pharynx region.
A&C. Anterior pharyngeal region of reproductively-developing animals.
B&D. Anterior pharyngeal region of larvae arrested in dauer.
A. Anterior pharynx of an adult (horizontal TEM section). (Image source: N533 [D. Hall] J2 F300.) Inset, illustration of anterior pharyngeal muscles, showing the stacked-cylinder arrangement of pm1, pm2 and pm3. Only two pm3 cells are shown; the third pm3 cell is behind the lumen out of the plane of view. The lumen is depicted as a central cylinder. When the pharyngeal muscles are relaxed, the lumen is closed.
B. Horizontal TEM section of anterior pharynx in a dauer. (Image source: Starved N2 dauer 50-7-1 [D. Riddle] 34.)
C. Anterior pharynx of an L2 (transverse TEM section). (Image source: N2 L2 28-14 [D. Riddle] 144.)
D. Anterior pharynx of a dauer (transverse TEM section). (Image source: Starved N2 dauer 50-2-2 [D. Riddle] 225.) Karets indicate hemi-desmosomes anchoring pm1 muscle fibers to the basal lamina. Cytoplasmic intermediate filaments (IF) can be detected in the pharyngeal epithelial cell cytoplasm adjacent to the pm1 cells. The lumen of the nondauer animals contain ingested bacterial cells as evidence of feeding.
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