AIntroFIG 4: A single worm imaged at time points throughout lifespan.
Brightfield images are shown of a single individual, for each day of life from the L4-adult molt (top image) until death (bottom image). Images were acquired at 10× magnification, using a custom culture apparatus maintained at 25°C. The position of the individual in each image was manually annotated and the images were computationally straightened into the "worm frame of reference" with anterior on the right and posterior on the left. Shrinking of the animal with age is particularly striking in this series. (Image source: Z. Pincus, Washington University; Zhang et al., 2016.).)
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